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METHODS OF MANUFACTURING HYDROGEL MICROPARTICLES HAVING LIVING CELLS, AND COMPOSITIONS FOR MANUFACTURING A SCAFFOLD FOR TISSUE ENGINEERING
METHODS OF MANUFACTURING HYDROGEL MICROPARTICLES HAVING LIVING CELLS, AND COMPOSITIONS FOR MANUFACTURING A SCAFFOLD FOR TISSUE ENGINEERING
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机译:具有活细胞的水凝胶微颗粒的制备方法,以及用于组织工程的脚手架的组合物
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摘要
Method of manufacturing hydrogel microparticles comprising one or more species of living cells attached thereon and/or encapsulated therein is provided. The method includes dissolving a hydrogel-forming agent in an aqueous medium to form a solution; suspending one or more species of living cells in the solution to form a cell suspension; dispersing the cell suspension into an organic oil to form a microemulsion; and subjecting the microemulsion to conditions that allow the hydrogel-forming agent to form hydrogel microparticles comprising one or more species of living cells attached thereon and/or encapsulated therein. Composition comprising a mixture of a degradable hydrogel and at least one hydrogel microparticle having one or more species of living cells, and method of manufacturing a scaffold for tissue engineering are also provided.
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