Method for the simultaneous detection of bacteria and fungi in a biological preparation by PCR, primers as well as bacteria and fungus detection kit

摘要

Method for the detection of bacteria and fungi in a sample of biological material in which the DNA contained in the sample of biological material is subjected to amplification in multiplex PCR in real time, in which the amplification reaction is carried out in two phases with the use of specific primers for bacteria and specific primers for fungi in the first phase, and then the product of the first multiplex amplification is used as a template in the second phase - amplification using primers and probes that differentiate fungi in a group of fungi of mold and yeast fungi and bacteria in Gram-positive and Gram-negative bacteria.