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METHODS AND REAGENTS FOR GENOTYPING HCV

机译:HCV基因分型的方法和试剂

摘要

The present invention is directed to methods and reagents for determining the genotype of a hepatitis C virus (HCV) species present in a test sample. The invention more particularly relates to mixtures of degenerate amplification and sequencing primers, and methods of using such primers, that are complementary to a plurality of HCV species, and are capable of generating nucleotide sequence information for a region of NS5B of HCV that is, for each species, indicative of the type and/or subtype, of the species present in the sample.
机译:本发明涉及用于确定测试样品中存在的丙型肝炎病毒(HCV)种类的基因型的方法和试剂。本发明更具体地涉及简并扩增引物和测序引物的混合物,以及使用此类引物的方法,所述引物与多种HCV种类互补,并能够产生HCV的NS5B区域的核苷酸序列信息,即指示样品中存在的物种的类型和/或亚型的每个物种。

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