CORN TRANSFORMATION EVENT AND SPECIFICITY IDENTIFICATION METHOD AND USE THEREOF

摘要

Disclosed are a corn transformation event and a specificity identification method and use thereof, wherein the transformation event uses the nucleotide sequences shown by SEQ ID NO.1 as the left wing region of the exogenous gene and uses the nucleotide sequences shown by SEQ ID NO.3 as the right wing region of the exogenous gene, or uses the nucleotide sequences shown by SEQ ID NO.14 as the left wing region of the exogenous gene and use the nucleotide sequences shown by SEQ ID NO.15 as the right wing region of the exogenous gene; the corn transformation event can introduce the exogenous gene specifically into a corn strain and endow the acceptor corn with insect-resistant and glyphosate-resistant capabilities; the insect-resistant and glyphosate-resistant gene in the acceptor corn can be stably inherited; and the expression of the insect-resistant and glyphosate-resistant gene would not produce an adverse impact on the agronomic traits of the acceptor corn, and implementing the identification method can provide molecular markers for using the transformation event to breed, thereby increasing the efficiency of the breeding work.