FLUORESCENCE DETECTION PRIMER, DETECTION METHOD, AND DETECTION KIT FOR CULEX WOLBACHIA

摘要

A fluorescence detection primer, detection method, and detection kit for Culex Wolbachia. The fluorescence detection primer is: wPipF: 5'-GTTTGTGCAGCTAATAG-3'; wPipR: 5'-GTCTGCAAGGCCTATTTCTACTG-3'; probe: 5'-CTTTCAATTGAAAAGATTCGATCAAC-3'; the two ends of the probe are respectively combined with a fluorescence generation group and a fluorescence quenching group. Culex Wolbachia can be detected by utilizing the fluorescence detection primer and the probe of the present invention on the basis of the detection method. In the method, the lowest detection limit is 100 copies/ml, and no amplification of Aedes Wolbachia occurs.