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Library preparation of tagged nucleic acid using single tube add-on protocol
Library preparation of tagged nucleic acid using single tube add-on protocol
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机译:使用单管附加方案制备标记核酸的文库
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摘要
A method of preparing a library of tagged nucleic acid fragments including contacting a population of cells directly with a lysis reagent having one or more protease to generate a cell lysate; inactivating the protease to generate an inactivated cell lysate, and applying a transposase and a transposon end composition containing a transferred strand to the inactivated cell lysate under conditions wherein the target nucleic acid and the transposon end composition undergo a transposition reaction.
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