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Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIs)
Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIs)
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机译:使用具有独特分子指数(UMI)的冗余读段抑制测序DNA片段中的错误
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摘要
The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index (UMI) sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the UMIs include both physical (exogenous) UMIs, e.g. introduced using Y-shaped adaptors, and virtual (endogenous) UMIs present in the DNA fragments to be sequenced. In some implementations, the unique molecular index sequences include non-random sequences. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.
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