METHODS FOR THE GENERATION OF MULTIPLE ORDERED NEXT-GENERATION SEQUENCING READS ALONG LARGE SINGLE DNA MOLECULES

摘要

Methods are provided for producing long-range DNA scaffold sequence templates by generating multiple ordered next-generation sequencing short reads on large single DNA molecules, for genome sequence assembly and analysis. The methods described herein pursue cost-effective and massively parallel ways to produce long-range sequence information for large and complex genome sequence assembly and analysis, such as de novo genome sequencing, haplotype-phasing, long-range genome structure variation detection, high-resolution metagenomics, and rapid genotyping for disease diagnosis and important trait-genetic association etc.