首页> 外国专利> A METHOD OF PRODUCING LI G NOCELL 11 LOL YT I C ENZYMES USING A SPECIES OF WHITE ROT FUNGI BY SOUP STATE FERMENTATION (SSF)

A METHOD OF PRODUCING LI G NOCELL 11 LOL YT I C ENZYMES USING A SPECIES OF WHITE ROT FUNGI BY SOUP STATE FERMENTATION (SSF)

机译:一种利用白腐真菌通过菌种发酵生产李格诺菌11 L酶的方法

摘要

The embodiments of the present invention relate to a method of producing lignocellulolytic enzymes using a species of white rot fungi by solid stale fermentation (SSF) is provided. The method comprises obtaining artificially modified fungus. The artificially modified fungus is Trammetes lactinea (GanoBF I ). An inoculum is prepared using said artificially modified fungus. A substrate is prepared using rubber-wood sawdust, vermiculite and rice bran. The said substrate is inoculated with the said prepared inoculum. The substrate is incubated to obtain the lignocellulolytic enzymes. The incubation is done tn a growth chamber at 25 ± 2°C under SSF static conditions. The fermentation is carried out of the said incubated substrate for at least 21 days.
机译:本发明的实施方式涉及提供一种通过固体陈旧发酵(SSF)使用一种白腐真菌来生产木质纤维素分解酶的方法。该方法包括获得人工修饰的真菌。人工修饰的真菌是 Trammetes lactinea (GanoBF I)。使用所述人工修饰的真菌制备接种物。使用橡胶木锯末,ver石和米糠制备基材。用所述制备的接种物接种所述底物。孵育底物以获得木质纤维素分解酶。在SSF静态条件下,在25±2°C的生长室中进行孵育。从所述温育的底物进行发酵至少21天。

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