ANTIBODY SUBJECTED TO FLUORESCENT LABELING USING NUCLEOTIDE BINDING SITE (NBS) OF ANTIBODY

摘要

Provided are: a method for using a nucleotide binding site present in the variable domain of the antibody to prepare, through a photochemical modification method, a fluorescent labeled antibody in which the fluorescence intensity changes with the binding of an antigen; and a fluorescent labeled antibody obtained thereby. A labeled antibody for fluoroimmunoassay in which a compound for labeling a nucleotide binding site (NBS) of the antibody and having indole butyric acid (IBA) and a fluorescent group bound thereto is conjugated to a nucleotide binding site in the antibody. The compound is represented by formula (1) (in the formula, F is a fluorescent group, and R is a linking group selected from the group consisting of an alkyl group, alkenyl group, alkynyl group, C4–C10 cycloalkyl group, C4–C10 heterocyclyl group, C4–C10 aryl group, C4–C10 heteroaryl group, arylene group, ether group, ester group, PEG group, sulfide group, amide group, ketone group, and sulfamide group, the groups being substituted or unsubstituted, and combinations thereof).