METHODS TO ISOLATE EXTRACELLULAR VESICLES IN BIOFLUIDS FOR BIOMARKER DISCOVERY AND DISEASE DIAGNOSIS

摘要

EVs are connected to onset and progression of numerous diseases and can be used for their diagnosis. Methods and platform technology to isolate EVs and detect intravesicle phosphoproteins from biofluids are discussed. Secreted extracellular vesicles in biofluids are effectively isolated and phosphoproteins are extracted for detection and identification as biomarkers for disease diagnosis using antibodies, mass spectrometry, or other like methods.