METHOD OF SYNTHESIZING AND SECRETING BLACK WIDOW SPIDER DRAGLINE SILK PROTEIN UTILIZING SILKWORM SILK GLAND BIOREACTOR

摘要

Provided is a method of synthesizing and secreting a black widow spider dragline silk protein utilizing a silkworm silk gland bioreactor. The method comprises: through transgenesis breeding a silkworm variety homozygous for a black widow spider dragline silk protein gene and capable of synthesizing and secreting a black widow spider dragline silk protein, the silkworm variety being a transgenic silkworm capable of synthesizing and secreting black widow spider dragline silk protein I, or a transgenic silkworm capable of synthesizing and secreting black widow spider dragline silk protein II, or a hybrid of the above two silkworm varieties. A silkworm silk gland cell of the above silkworm variety synthesizes and secretes a black widow spider dragline silk protein, and the protein is introduced into a cocoon with the silk-spinning and cocoon-forming behavior of the silkworm.