FLUORESCENT PROBE FOR DETECTING EXTRACELLULAR METABOLITE AND SCREENING METHOD EMPLOYING SAID FLUORESCENT PROBE

摘要

[Problem] The present invention addresses the problem of developing a novel fluorescent probe that allows high-sensitivity detection of lactic acid, which is a substance released outside cells as the final product of the glycolytic system, and providing a screening method with which it is possible, by using such an evaluation system, to search for compounds that exhibit inhibitory effects on the glycolytic system and that are candidate anti-cancer agents. [Solution] Provided is a fluorescent probe for detecting NADH containing that contains a compound represented by formula (I) or a salt thereof [in the formula, R¹ represents the same or different types of 1-5 water-soluble substituents; R² represents the same or different types of 1-5 electron-accepting substituents; each of R³, R⁴, R⁵, and R⁶ independently represents a hydrogen atom or an alkyl group; each of R⁷ and R⁸ independently represents a hydrogen atom or the same or different types of 1-3 substituents that are independently selected from a group including a hydroxy group, a halogen atom, alkyl groups each of which may include substitution, a sulfo group, a carboxy group, an ester group, an amide group, and an azido group; and each of R^a and R^b independently represents a hydrogen atom or an alkyl group, wherein, in the case in which R³ or R⁴ is an alkyl group, an annular structure may be formed together with R⁷, which includes nitrogen atoms with which these groups are bonded, and, in the case in which R⁵ or R⁶ is an alkyl group, an annular structure may be formed together with R⁸, which includes nitrogen atoms with which these groups are bonded.].