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MICRO- AND NANOCONTACT PRINTING WITH AMINOSILANES: PATTERNING SURFACES OF MICROFLUIDIC DEVICES FOR MULTI- PLEXED BIOASSAYS
MICRO- AND NANOCONTACT PRINTING WITH AMINOSILANES: PATTERNING SURFACES OF MICROFLUIDIC DEVICES FOR MULTI- PLEXED BIOASSAYS
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机译:氨基硅酮的微接触和纳米接触印刷:用于多生物测定的微流体装置的表面图案化
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摘要
It is an object of the present invention to achieve rapid surface patterning of biomolecules within microfluidic devices with high reproducibility. In this work, we present a new means of creating micro- and nano-patterns of aminosilanes within microfluidic devices via an aqueous based microcontact printing technique. To minimize the diffusion of molecules into the PDMS stamp, we use water as the inking solvent and enforce short incubation and contact times during the printing process to preserve the pre-defined resolution of patterned features. These patterns then serve as the building block to couple multiple biomolecules in solution onto a single surface for subsequent bioassays.
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