An SEM-EDX method for detecting distribution characteristics of nitrogen, phosphorus and potassium in a crop leaf. Surface scanning and line scanning in the direction of thickness are carried out in the cross section of the leaf by employing a combination of a scanning electron microscope (SEM) with energy dispersive X-ray spectroscopy (EDX) to obtain an energy spectrum diagram of nitrogen, phosphorus and potassium in the leaf, and then the energy spectrum diagram is analyzed to obtain the space distribution characteristics of nitrogen, phosphorus and potassium in a mesophyll tissue. Nitrogen, phosphorus and potassium are distributed throughout the cross section of the leaf. Nitrogen and phosphorus have similar distribution characteristics, they are both distributed with significantly higher densities along the periphery of the cell wall contour than other sites; and potassium is distributed relatively uniformly throughout the cross section of the leaf. The above-mentioned detection method solves the problem that the space distribution characteristics of nitrogen, phosphorus and potassium in the leaf cannot be determined, and can be applied to the microscopic detection of crop nutrients.
展开▼
