Provided are an analysis vessel in which a liquid is allowed to flow in a controlled manner, a target analysis device employing the same, and a target analysis method. This analysis vessel is characterized by comprising a first chamber 11, a second chamber 12 and a third chamber 13, and in that: the first chamber 11, the second chamber 12 and the third chamber 13 are sequentially arranged in this order; the analysis vessel has a first partition 111 between the first chamber 11 and the second chamber 12; the analysis vessel has a second partition 121 between the second chamber 12 and the third chamber 13; a sample holding instrument can be inserted inside the first chamber 11 from the outside; the first partition wall 111 breaks as a result of coming into contact with the tip of the sample holding instrument, which has been inserted in the first chamber 11; the second partition 121 is a porous partition; the third chamber 13 has at least one through hole 14; an openable/closable closing member is disposed on the through hole 14 to cover the through hole; and the closing member is removed to open the through hole 14 in order to allow liquid to flow from the second chamber 12 to the third chamber 13.
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