FLUORESCENT PROBE, FLUORESCENCE DETECTION METHOD, AND METHOD FOR USING FLUORESCENT PROBE

摘要

The present invention relates to a fluorescent probe which includes: a carrier molecule; and fluorescent dye a and fluorescent dye b which are bonded to the carrier molecule. Fluorescent dye a and fluorescent dye b have different excitation wavelengths. Fluorescence resonance energy transfer (FRET) does not occur between fluorescent dye a and fluorescent dye b. The present invention also relates to a fluorescence detection method which includes: a step in which target cells are labelled with the fluorescent probe; and a step in which the target cells labelled with the fluorescent probe are irradiated with excitation light, and fluorescence from the fluorescent probe is observed. Furthermore, the present invention relates to a method for using the fluorescent probe, said method including: a step in which cells are fluorescence labelled using the fluorescent probe; a step in which the fluorescence-labelled cells are screened using a flow cytometer or a fluorescence microscope; a step in which the screened fluorescence-labelled cells are transplanted into a living organism; and a step in which the fluorescence-labelled cells in the living organism are observed using an in vivo fluorescence imaging device.