Nonylphenol responsive genes in Oryzias javanicus and the method for diagnosing aquatic environment pollution using the same

摘要

The present invention relates to an Oryzias javanicus gene responsive to nonylphenol (NP) exposure, and to a method for diagnosing aquatic environmental contamination by using the same. In particular, Oryzias javanicus is cultured, and is exposed to nonylphenol at a concentration of 20 g/L for 12 hours, 24 hours, 48 hours, and 72 hours, and RNA is separated therefrom. Then, cDNA is synthesized and marked by Cy3 and Cy5 before performing hybridization. An oligo-microarray is produced and analyzed by utilizing an EnviHaz-Fish array. As a result, it has been confirmed that an expression amount of 123 genes is changed by twice or more compared to a control group by the exposure of nonylphenol, using heterogeneous microarray experimental analysis. Accordingly, the genes can be useful as biomarkers for confirming nonylphenol exposure.