首页> 外国专利> Primer set for detecting Fusarium species which produce fusaric acid and detecting method using the same

Primer set for detecting Fusarium species which produce fusaric acid and detecting method using the same

机译：用于检测产镰孢酸的镰刀菌种的引物组及其检测方法

页面导航

摘要
著录项
相似文献

摘要

The present invention relates to a primer for detection of fumarium, and a method for detecting fumarium genus contained in agricultural products and the like using the primer. By using the primer pair according to the present invention,FusariumOf the species belonging to the genus Fusarium (fusaric acid)Fusarium) Can be detected within a short period of time with excellent efficiency in a species-specific manner.;

机译：本发明涉及用于检测primer的引物，以及使用该引物检测农产品等中所含的的属的方法。通过使用根据本发明的引物对，可以在短时间内用Fusarium（富铁酸）（I> Fusarium（I））属中的 Fusarium 以特定物种的方式具有出色的效率; 展开▼

著录项

公开/公告号KR101719769B1

专利类型

公开/公告日2017-03-28

原文格式PDF

申请/专利权人 대한민국;
展开▼

申请/专利号KR20150107219

发明设计人 홍성기;이데레사;이수형;함현희;류재기;
展开▼

申请日2015-07-29

分类号C12Q1/68;

国家 KR

入库时间 2022-08-21 13:25:48

相似文献

专利

外文文献

中文文献

1. 用于检测禾谷镰孢的引物组合和检测方法 [P] . 中国专利： CN110373412A . 2019-10-25

2. 用于分析大豆镰孢根腐真菌多样性的特异性引物组、试剂盒及其应用 [P] . 中国专利： CN106755416B . 2018.10.30

3. Primer set for detecting Fusarium species which produce fusaric acid and detecting method using the same [P] . 韩国专利： KR20170014230A . 2017-02-08

机译：用于检测产镰孢酸的镰刀菌种的引物组及其检测方法

4. PRIMER SET FOR DETECTING TYPE-B TRICHOTHECENE-PRODUCING ALLIED SPECIES AND FUSARIUM SPECIES AND DETECTION METHOD USING SAME [P] . 韩国专利： KR20170046924A . 2017-05-04

机译：用于检测产生b型三叶草烯的相关物种和枯萎菌属物种的底漆组及其检测方法

5. PRIMER SET FOR AMPLIFYING TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, PROBE OR PROBE SET SPECIFICALLY HYBRIDIZING WITH TARGET SEQUENCE(S) OF ANTIBIOTIC-RESISTANT BACTERIAL SPECIES, METHOD OF DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES USING THE PROBE OR PROBE SET, AND KIT FOR DETECTING ANTIBIOTIC-RESISTANT BACTERIAL SPECIES [P] . 美国专利： US2009163382A1 . 2009-06-25

机译：用于扩增抗生细菌物种，探针或探针的目标序列的杂交蛋白，特别是与抗生细菌物种的靶序列进行杂交，检测抗细菌序列的方法和试剂盒，用于检测抗细菌细菌

1. Specificity of Various Mitochondrial DNA (mtDNA), ND5, D-Loop, and Cty-bDNA Primers in Detecting Pig (Sus scrofa) DNA Fragments [J] . Joni Kusnadi, Noval Audi Ashari, Estri Laras Arumingtyas 美国分子生物学期刊（英文） . 2020,第003期

2. Biocontrol traits of two Paenibacillus polymyxa strains SQR-21 and WR-2 in response to fusaric acid, a phytotoxin produced by Fusarium species [J] . Raza W., Yuan J., Wu Y. C., Plant Pathology . 2015,第5期

3. Toxins Produced by Fusarium Species in Leaf Scorch Decline Affected Coconut Palms (Cocos nucifera L.): Quantitative Analysis of Fusaric Acid, Zearalenone and T-2 [J] . P.H.A.P. Siriwardhana, L.C.P. Fernando, J.M.M.A. Jayasundara Cord . 2009,第1期

4. Comparison of the production of fusaric acid produced by Fusarium oxysporum f. sp. lycopersici in different cultures with HPLC method [J] . S. Pirayesh, H. Zamanizadeh, B. Morid International Journal of Biosciences . 2015,第3期

5. Development of Real-Time PCR Primer and Probe Sets for Detecting Degenerated and Non-degenerated Forms of the Butanol-Producing Bacterium Clostridium acetobutylicum ATCC 824 [C] . Sun-Mi Lee, Min Ok Cho, Youngsoon Um, Symposium on biotechnology for fuels and chemicals . 2010

6. Development and application of multiple polymerase chain reaction assays to detect ochratoxin-producing Aspergillus and Penicillium, and trichothecene-producing Fusarium in stored and processed grain. [D] . Boddeda, Anuradha. 2008

7. A relative quantitative method to detect OCT4A gene expression by exon-junction primer and locked nucleic acid-modified probe [O] . Jian-jun Ren, Xing-kai Meng 2011

8. Rapid Detection Method for Fusaric Acid-producing Species of Fusarium by PCR [O] . Theresa Lee, Sosoo Kim, Mark Busman, 2015

1. Application of polymerase chain reaction-sequence specific primer method in detecting multiple genotypes based on a large sample of mental diseases [J] . Yang Rui ,Song Xiao-rong ,Qin Chuan . 中国组织工程研究与临床康复 . 2007,第34期

2. The effects of neem Azadirachta indica A. Juss (meliaceae) oil on Fusarium oxysporum f. sp. medicagenis and Fusarium subglutinans and the production of fusaric acid toxin [J] . Marcia Regina Ferreira Geraldo ,Carla Cristina Arroteia ,Carlos Kemmelmeier . 生命科学与技术进展（英文） . 2010,第1期

3. USING　3　PRIMER　PAIRS　TO　DETECT　HBV　DNA　IN　LIVERTISSUES　FROM　HEPATOCELLULAR　CARCINOMASWITH　PCR　TECHNIQUE [J] . 陈明 ,吕凌 ,姚集鲁 . 中国癌症研究：英文版 . 1996,第1期

4. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

5. 重庆地区玉米穗腐病拟轮枝镰孢及禾谷镰孢产毒性检测 [C] . 周丹妮 ,王晓鸣 ,陈国康 . 国家真菌毒素防控科技创新联盟成立大会暨第一届中国真菌毒素大会 . -1

6. 亚细亚镰孢、假禾谷镰孢、接骨木镰孢LAMP检测技术的构建及江苏省小麦赤霉病菌种群组成分析 [A] . 许苗 . 2018