SiNG-PCRseq A method of standarized nucleic acid quantitation through spiking-in neighbor genome-coupled competitive PCR amplicon sequencing

摘要

The present invention relatives after mixing the dielectric for the determination target sample utilizing the similarity between closely related species sequence by co-amplification (co-amplification) Competitive PCR amplicon was sequenced (piking- si n gn eighbor enome-coupled competitive P CR amplicon seq uencing, SiNG-PCRseq) method to apply, to the sample on the inside nucleic acid quantitative analysis method, and specifically SiNG-PCRseq method of the present invention is the relative DNA standards in various experimental conditions that may vary, such as cell lines, polymerase may provide a quantitative value, the provided standardized DNA quantification value, regardless of the existing nucleic acid quantitative when analysis compared with RNA-seq methods used, the sample for the relative quantification value for my nucleic experimental conditions and sample types Since accurate, easy, SiNG-PCRseq method of the present invention can be useful in standard quantitative analysis of nucleic acid in a sample method.;