首页> 外国专利> INS INSulin gene knockout diabetes mellitus or diabetic complications animal model and a method for producing the same

INS INSulin gene knockout diabetes mellitus or diabetic complications animal model and a method for producing the same

机译：INS胰岛素基因基因敲除糖尿病或糖尿病并发症动物模型及其制备方法

页面导航

摘要
著录项
相似文献

摘要

The present invention relates to an animal model of INS gene knockout diabetes or diabetic complication and a method for producing the same. More particularly, the present invention relates to a DNA sequence encoding a small guide RNA (sgRNA) and a recombinant protein for producing a diabetic or diabetic complicated animal model The present invention relates to an animal model of diabetes mellitus or diabetic complication and a method for producing the same. The recombinant vector using the specific sgRNA and the CRISPR / Cas9 method for knocking out the INS gene according to the present invention can be used for transforming the DNA strand encoding the INS gene in the transformed animal in whole or in part to prevent diabetes, Can be effectively induced and can accompany diabetes complications. It is very useful to use an animal model of diabetic or diabetic complication according to the present invention which enables long-term studies for the practical prevention or treatment of diabetes mellitus, which is suffering from diabetes mellitus and complications. Therefore, the animal model of INS gene knockout diabetes or diabetic complication according to the present invention can be used variously to identify the mechanism of the related diseases, search for therapeutic substances, and develop diagnostic methods.

机译：本发明涉及 INS 基因敲除型糖尿病或糖尿病并发症的动物模型及其制备方法。更具体地，本发明涉及编码小指导RNA（sgRNA）的DNA序列和用于产生糖尿病或糖尿病复杂动物模型的重组蛋白。本发明涉及糖尿病或糖尿病并发症的动物模型及其制备方法。生产相同的。根据本发明，使用特异性sgRNA和CRISPR / Cas9方法敲除 INS 基因的重组载体可用于转化编码 INS 基因的DNA链。在转化的动物中，全部或部分预防糖尿病，可以有效地诱发并可以伴随糖尿病并发症。使用根据本发明的糖尿病或糖尿病并发症的动物模型是非常有用的，该动物模型使得能够长期研究以实际预防或治疗患有糖尿病和并发症的糖尿病。因此，根据本发明的 INS 基因敲除糖尿病或糖尿病并发症的动物模型可用于鉴定相关疾病的机理，寻找治疗物质并开发诊断方法。 展开▼

著录项

公开/公告号KR101748575B1

专利类型

公开/公告日2017-06-20

原文格式PDF

申请/专利权人 MGENPLUS CO. LTD.;
展开▼

申请/专利号KR20160172849

发明设计人 박광욱;강정택;조범래;김수진;
展开▼

申请日2016-12-16

分类号C12N15/85;A01K67/027;C12N9/22;G01N33/50;

国家 KR

入库时间 2022-08-21 13:25:18

相似文献

专利

外文文献

中文文献

1. 可用于预防患有胰岛素耐受的前驱糖尿病患者的2型糖尿病及其并发症的组合物 [P] . 中国专利： CN101663030B . 2013.08.21

2. 可用于预防患有胰岛素耐受的前驱糖尿病患者的2型糖尿病及其并发症的组合物 [P] . 中国专利： CN101663030A . 2010-03-03

3. Derivatives of amidocarboxylic acid, agents for lowering blood glucose, reducing lipid, for improving insulin resistance, anti-inflammatory, for immuno-regulation, for inhibiting aldose reductase, for inhibiting 5-lipoxygenase, for the generation of suppressor of lipid peroxide, to activate ppar, to reduce osteoporosis, and compositions for the therapy or prevention of diabetes mellitus, hyperlipemia, obesity, impaired glucose tolerance, insulin resistance other than igt, liver fatty, diabetic complications, arteriosclerosis, gestational diabetes mellitus, polycystic ovary syndrome, arthroesthesis, rheumatoid arthritis, allergic diseases, asthma, cancer, autoimmune diseases, pancreatitis, and cataracts [P] . BR9813019A . 2000-09-05

机译：酰胺基羧酸的衍生物，用于降低血糖，减少脂质，改善胰岛素抵抗，消炎，用于免疫调节，用于抑制醛糖还原酶，用于抑制5-脂氧合酶，用于产生脂质过氧化物的抑制剂，激活ppar，以减少骨质疏松症，以及用于治疗或预防糖尿病，高脂血症，肥胖症，糖耐量减低，胰岛素抵抗的胰岛素组合物，肝脂肪，糖尿病并发症，动脉硬化，妊娠糖尿病，多囊卵巢综合征，关节置换，类风湿病的组合物关节炎，过敏性疾病，哮喘，癌症，自身免疫性疾病，胰腺炎和白内障

4. ANIMAL MODEL OF DIABETES WITH INS GENE KNOCKOUT OR DIABETIC COMPLICATIONS AND MANUFACTURING METHOD THEREOF [P] . 世界知识产权组织专利： WO2018110805A1 . 2018-06-21

机译：具有基因敲除或糖尿病并发症的糖尿病动物模型及其制造方法

5. COMPOSITIONS AND METHODS FOR INDUCING SATIETY AND TREATING NON-INSULIN DEPENDENT DIABETES MELLITUS, PRE-DIABETIC SYMPTOMS, INSULIN RESISTANCE AND RELATED DISEASE STATES AND CONDITIONS [P] . 美国专利： US2018369177A1 . 2018-12-27

机译：诱导饱食感和治疗非胰岛素依赖性糖尿病，糖尿病前症状，胰岛素抵抗和相关疾病状态和条件的组合物和方法

1. Insulin Sensitivity and Insulin Secretion Estimated by Homeostatic Model Assessment (HOMA) in Gestational Diabetes Mellitus [J] . Samira Mahjabeen, A. K. M. Shahidur Rahman, Mahmudul Hossain, 生物科学与医学（英文） . 2020,第007期

2. RELATION OF HYPERTENSION TO DIABETIC NEPHROPATHY IN PATIENTS WITH NON INSULIN DEPENDENT DIABETES MELLITUS-A PAIR MATCHED CASE CONTROL STUDY [J] . 侯旭宏, 王建华, 冯凭中国医学科学杂志（英文版） . 2002,第001期

3. Study　of　the　Pathogenesis　of　Hypertension　Associated　with　Non-Insulin　Dependent　Diabetes　Mellitus [J] . 丁国宪, 陈家伟, 何戎华生物医学研究杂志：英文版 . 1997,第002期

4. EFFECTS OF JIANG TANG FANG LONG FORMULA ON INSULIN PRODUCTION AND FUNCTION IN AN ANIMAL MODEL OF DIABETIC HEARING LOSS [J] . YANG Yunxia, LI Ruiyu, JING Jianmei, 中华耳科学杂志（英文版） . 2013,第002期

5. Antihypertensive effect of insulin in the zucker diabetic fatty (ZDF) rat, an animal model for non-insulin dependent diabetes mellitus with insulin resistance [J] . MIKIKO KAWAGUCHI, KUNIO KOSHIMURA, YOSHIO MURAKAMI, Biomedical Research . 1999,第1期

6. Diabetic Autonomic Neuropathies as Complications of Insulin and Non-insulin Dependent Diabetes Mellitus [J] . Akhil.R, Karthik.N, Iyyappan.D, Advanced Research in Electrical and Electronic Engineering: AREEE . 2015,第6期

7. Diabetic Autonomic Neuropathies as Complications of Insulin and Non-insulin Dependent Diabetes Mellitus [J] . Akhil.R, Karthik.N, Iyyappan.D, International Journal of Basic and Applied Biology: IJBAB . 2015,第6期

8. Insulin-Coenzyme Q_(10) coencapsulated PLGA nanoparticles for oral delivery: A better regimen for treatment of diabetes mellitus and its complications [C] . G. Sharma, D. V. Ratnam, G. Mittal, Controlled Release Society Annual Meeting and Exposition . 2007

9. Characterization of neonatal porcine islets as an alternative source of insulin-producing tissue for transplantation into patients with insulin-dependent diabetes mellitus. [D] . Rayat, Gina Ranile. 1999

10. Development of non-insulin-dependent diabetes mellitus in the double knockout mice with disruption of insulin receptor substrate-1 and beta cell glucokinase genes. Genetic reconstitution of diabetes as a polygenic disease. [O] . Y Terauchi, K Iwamoto, H Tamemoto, 1997

11. Poster session 2Morphogenetic mechanisms290MiR-133 regulates retinoic acid pathway during early cardiac chamber specification291Bmp2 regulates atrial differentiation through miR-130 during early heart looping formationDevelopmental genetics294Association of deletion allele of insertion/deletion polymorphism in alpha 2B adrenoceptor gene and hypertension with or without type 2 diabetes mellitus295Association of G1359A polymorphism of the endocannabinoid type 1 receptor (CNR1) with coronary artery disease (CAD) with type 2 diabetes mellitusCell growth, differentiation and stem cells - Vascular298Gamma-secretase inhibitor prevents proliferation and migration of ductus arteriosus smooth muscle cells: a role of Notch signaling in postnatal closure of ductus arteriosus299Mesenchymal stromal-like cells (MLCs) derived from induced pluripotent stem (iPS) cells: a promising therapeutic option to promote neovascularization300Sonic Hedgehog promotes mesenchymal stem cell differentiation to vascular smooth muscle cells in cardiovacsular disease301Proinflammatory cytokine secretion and epigenetic modification in endothelial cells treated LPS-GinfivalisCell death and apoptosis - Vascular304Mitophagy acts as a safeguard mechanism against human vascular smooth muscle cell apoptosis induced by atherogenic lipidsTranscriptional control and RNA species - Vascular307MicroRNA-34a role in vascular calcification308Local delivery of a miR-146a inhibitor utilizing a clinically applicable approach attenuates neointima formation after vascular injury309Long noncoding RNA landscape of hypoxic endothelial cells310Specific circulating microRNAs levels associate with hypertension, hyperglycemia and dysfunctional HDL in acute coronary syndrome patientsCytokines and cellular inflammation - Vascular313Phosphodiesterase5A up-regulation in vascular endothelium under pro-inflammatory conditions: a newly disclosed anti-inflammatory activity for the omega-3polyunsaturated aatty acid docosahexaenoic acid314Cardiovascular risk modifying with extra-low dose anticytokine drugs in rhematoid arthritis315Conversion of human M-CSF macrophages into foam cells reduces their proinflammatory responses to classical M1-polarizing activation316Lymphocytic myocarditis coincides with increased plaque inflammation and plaque hemorrhage in coronary arteries, facilitating myocardial infarction317Serum osteoprotegerin level predictsdeclined numerous of circulating endothelial- derived and mononuclear-derived progenitor cells in patients with metabolic syndromeGrowth factors and neurohormones - Vascular320Effect of gastrin-releasing peptide (GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325Regulation of beta-adrenoceptor-evoked inotropic responses by inhibitory G protein, adenylyl cyclase isoforms 5 and 6 and phosphodiesterases326Binding to RGS3 and stimulation of M2 muscarinic acetylcholine receptors modulates the substrate specificity of p190RhoGAP in cardiac myocytes327Cardiac regulation of post-translational modifications, parylation and deacetylation in LMNA dilated cardiomyopathy mouse model328Beta-adrenergic regulation of the b56delta/pp2a holoenzyme in cardiac myocytes through b56delta phosphorylation at serine 573Nitric oxide and reactive oxygen species - Vascular331Oxidative stress-induced miR-200c disrupts the regulatory loop among SIRT1, FOXO1 and eNOS332Antioxidant therapy prevents oxidative stress-induced endothelial dysfunction and Enhances Wound Healing333Morphological and biochemical characterization of red blood cell in coronary artery diseaseCytoskeleton and mechanotransduction - Heart336Novel myosin activator, JSH compounds, increased myocardial contractility without chronotropic effect in ratsExtracellular matrix and fibrosis - Vascular339Ablation of Toll-like receptor 9 causes cardiac rupture after myocardial infarction by attenuating proliferation and differentiation of cardiac fibroblasts340Altered vascular remodeling in the mouse hind limb ischemia model in Factor VII activating protease (FSAP) deficiencyVasculogenesis, angiogenesis and arteriogenesis343Pro-angiogenic effects of proly-hydroxylase inhibitors and their potential for use in a novel strategy of therapeutic angiogenesis for coronary total occlusion344Nrf2 drives angiogenesis in transcription-independent manner: new function of the master regulator of oxidative stress response345Angiogenic gene therapy, despite efficient vascular growth, is not able to improve muscle function in normoxic or chronically ischemic rabbit hindlimbs -role of capillary arterialization and shunting346Effect of PAR-1 inhibition on collateral vessel growth in the murine hind limb model347Quaking is a key regulator of endothelial cell differentiation, neovascularization and angiogenesis348"Emerging angiogenesis" in the chick chorioallantoic membrane (CAM). An in vivo study349Exosomes from cardiomyocyte progenitor cells and mesenchymal stem cells stimulate angiogenesis in vitro and in vivo via EMMPRINEndothelium352Reciprocal regulation of GRK2 and bradykinin receptor stimulation modulate Ca2+ intracellular level in endothelial cells353The roles of bone morphogenetic proteins 9 and 10 in endothelial inflammation and atherosclerosis354The contribution of GPR55 to the L-alpha-lysophosphatidylinositol-induced vasorelaxation in isolated human pulmonary arteries355The endothelial protective ACE inhibitor Zofenoprilat exerts anti-inflammatory activities through H2S production356A new class of glycomimetic drugs to prevent free fatty acid-induced endothelial dysfunction357Endothelial progenitor cells to apoptotic endothelial cell-derived microparticles ration differentiatesas preserved from reduced ejection fractionheart failure358Proosteogenic genes are activated in endothelial cells of patients with thoracic aortic aneurysm359Endothelin ETB receptors mediate relaxing responses to insulin in pericardial resistance arteries from patients with cardiovascular disease (CVD)Smooth muscle and pericytes362CX3CR1 positive myeloid cells regulate vascular smooth muscle tone by inducing calcium oscillations via activation of IP3 receptors363A novel function of PI3Kg on cAMP regulation, role in arterial wall hyperplasia through modulation of smooth muscle cells proliferation364NRP1 and NRP2 play important roles in the development of neointimal hyperplasia in vivo365Azithromycin induces autophagy in aortic smooth muscle cellsCoagulation, thrombosis and platelets368The real time in vivo evaluation of platelet-dependent aldosterone prothrombotic action in mice369Development of a method for in vivo detection of active thrombi in mice370The antiplatelet effects of structural analogs of the taurine chloramine371The influence of heparin anticoagulant drugs on functional state of human platelets372Regulation of platelet aggregation and adenosine diphosphate release by d dimer in acute coronary syndrome (in vitro study)Oxygen sensing, ischaemia and reperfusion375Sirtuin 5 mediates brain injury in a mouse model of cerebral ischemia-reperfusion376Abscisic acid: a new player in cardiomyocyte protection from ischaemia?377Protective effects of ultramicronized palmitoylethanolamide (PEA-um) in myocardial ischaemia and reperfusion injury in vivo378Identification of stem cell-derived cardiomyocytes using cardiac specific markers and additional testing of these cells in simulated ischemia/reperfusion system379Single-dose intravenous metformin treatment could afford significant protection of the injured rat kidney in an experimental model of ischemia-reperfusion380Cardiotoxicity of long acting muscarinic receptor antagonists used for chronic obstructive pulmonary disease381Dependence antioxidant potential on the concentration of amino acids382The impact of ischemia-reperfusion on physiological parameters,apoptosis and ultrastructure of rabbit myocardium with experimental aterosclerosisMitochondria and energetics385MicroRNA-1 dependent regulation of mitochondrial calcium uniporter (MCU) in normal and hypertrophied hearts386Mitochondrial homeostasis and cardioprotection: common targets for desmin and aB-crystallin387Overexpression of mitofusin-2 (Mfn2) and associated mitochondrial dysfunction in the diabetic heart388NO-dependent prevention of permeability transition pore (MPTP) opening by H2S and its regulation of Ca2+ accumulation in rat heart mitochondria389G protein coupled receptor kinase 2 (GRK2) is fundamental in recovering mitochondrial morphology and function after exposure to ionizing radiation (IR)Gender issues392Sex differences in pulmonary vascular control; focus on the nitric oxide pathwayAging395Heart failure with preserved ejection fraction develops when feeding western diet to senescence-accelerated mice396Cardiovascular markers as predictors of cognitive decline in elderly hypertensive patients397Changes in connexin43 in old rats with volume overload chronic heart failureGenetics and epigenetics400Calcium content in the aortic valve is associated with 1G>2G matrix metalloproteinase 1 polymorphism401Neuropeptide receptor gene s (NPSR1) polymorphism and sleep disturbances402Endothelin-1 gene Lys198Asn polymorphism in men with essential hypertension complicated and uncomplicated with chronic heart failure403Association of common polymorphisms of the lipoprotein lipase and pon1 genes with the metabolic syndrome in a sample of community participantsGenomics, proteomics, metabolomics, lipidomics and glycomics405Gene expression quantification using multiplexed color-coded probe pairs to determine RNA content in sporadic cardiac myxoma406Large-scale phosphorylation study of the type 2 diabetic heart subjected to ischemia / reperfusion injury407Transcriptome-based identification of new anti-inflammatory properties of the olive oil hydroxytyrosol in vascular endothelial cell under basal and proinflammatory conditions408Gene polymorphisms combinations and risk of myocardial infarctionComputer modelling, bioinformatics and big data411Comparison of the repolarization reserve in three state-of-the-art models of the human ventricular action potentialMetabolism, diabetes mellitus and obesity414Endothelial monocyte-activating polypeptide-II improves heart function in type -I Diabetes mellitus415Admission glucose level is independent predictor of impaired left ventricular function in patients with acute myocardial infarction: a two dimensional speckle-tracking echocardiography study416Association between biochemical markers of lipid profile and inflammatory reaction and stiffness of the vascular wall in hypertensive patients with abdominal obesity417Multiple common co-morbidities produce left ventricular diastolic dysfunction associated with coronary microvascular dysfunction, oxidative stress and myocardial stiffening418Investigating the cardiovascular effects of antiretroviral drugs in a lean and high fat/sucrose diet rat model of obesity419Statins in the treatment of non-alcoholic steatohepatitis (NASH). Our experience from a 2-year prospective study in Constanta County, Romania420Epicardial adipose tissue as a predictor of cardiovascular outcome in patients with ACS undergoing PCI?Arterial and pulmonary hypertension423Dependence between heart rhythm disorers and ID polymorphism of ACE gene in hypertensive patients424Molecular mechanisms underlying the beneficial effects of Urocortin 2 in pulmonary arterial hypertension425Inhibition of TGf-b axis and action of renin-angiotensin system in human ascending aorta aneurysms426Early signs of microcirculation and macrocirculation abnormalities in prehypertension427Vascular smooth muscle cell-expressed Tie-2 controls vascular tone428Cardiac and vascular remodelling in the development of chronic thrombo-embolic pulmonary hypertension in a novel swine modelBiomarkers431Arrhythmogenic cardiomyopathy: a new, non invasive biomarker432Can circulating microRNAs distinguish type 1 and type 2 myocardial infarction?433Design of a high-throughput multiplex proteomics assay to identify left ventricular diastolic dysfunction in diabetes434Monocyte-derived and P-selectin-carrying microparticles are differently modified by a low fat diet in patients with cardiovascular risk factors who will and who will not develop a cardiovascular event435Red blood cell distribution width assessment by polychromatic interference microscopy of thin films in chronic heart failure436Invasive and noninvasive evaluation of quality of radiofrequency-induced cardiac denervation in patients with atrial fibrillation437The effect of therapeutic hypothermia on the level of brain derived neurotrophic factor (BDNF) in sera following cardiopulmonary resustitation438Novel biomarkers to predict outcome in patients with heart failure and severe aortic stenosis439Biological factors linking depression and anxiety to cardiovascular disease440Troponins and myoglobin dynamic at coronary arteries graftingInvasive, non-invasive and molecular imaging443Diet composition effects on the genetic typing of the mouse ob mutation: a micro-ultrasound characterization of cardiac function, macro and micro circulation and liver steatosis444Characterization of pig coronary and rabbit aortic lesions using IV-OCT quantitative analysis: correlations with histologyGene therapy and cell therapy447Enhancing the survival and angiogenic potential of mouse atrial mesenchymal cells448VCAM-1 expression in experimental myocardial infarction and its relation to bone marrow-derived mononuclear cell retentionTissue engineering451Advanced multi layered scaffold that increases the maturity of stem cell-derived human cardiomyocytes452Response of engineered heart tissue to simulated ischemia/reperfusion in the presence of acute hyperglycemic conditions453Serum albumin hydrogels prevent de-differentiation of neonatal cardiomyocytes454A novel paintbrush technique for transfer of low viscosity ultraviolet light curable cyan methacrylate on saline immersed in-vitro sheep heart [O] . V Garcia-Martinez, C Lopez Sanchez, W Hamed, 2016

12. Effect of Intensive Treatment of Diabetes on the Development and Progression ofLong-Term Complications in Insulin-Dependent Diabetes Mellitus [R] . 1994

1. Gene therapy for type 1 diabetes mellitus in rats by gastrointestinal administration of chitosan nanoparticles containing human insulin gene [J] . Li Niu Yan-Cheng Xu Zhe Dai Hui-Qin Tang . 世界胃肠病学杂志：英文版 . 2008,第26期

2. 胰岛素抵抗与糖尿病慢性并发症的防治 [C] . . 首届国际中西医结合内分泌代谢病学术大会暨糖尿病论坛 . 2008

3. 重组腺相关病毒载体介导人类组织型激肽释放酶基因对2型糖尿病大鼠胰岛素抵抗和肾脏并发症影响的实验研究 [A] . 袁刚 . 2003