- Method for reprogramming cells using nano-carrier and producing induced pluripotent stem cells

摘要

The invention (a) silicon oxide (silicon dioxide) was coupled differentiation transcription factor inverse to the nanotube (nanotube) has a layer, a transcription factor comprising the steps of manufacturing the nanotube-carriers; Culturing with the nanotube carrier cells - (b) said transcription factor; And (c) embryonic stem cells from the culture obtained from (b) -, comprising a step of separating the similar colonies (embryonic stem cell-like colonies) it provides the inverse of the cell differentiation methods. Production process according to the present invention can essentially avoid the safety problems that may be caused by using a viral vector (e.g., progression potential of tumor cells, etc.). In addition, while preventing the denaturation of the protein, because the protein is continuously released from the nanotube-carriers, it is possible to supply a sufficient amount of protein without protein treatment repeatedly.;