PCR METHOD FOR RAPIDLY DETECTING ACTINOBACILLUS PLEUROPNEUMONIAE FROM CLINICAL SAMPLES THROUGH MULTIPLEX REAL-TIME PCR ASSAY AND THE APPARATUS THEREOF

摘要

The present invention relates to a method and apparatus for rapid detection of swine pleurisy pneumoniae from a clinical sample through multiple real-time PCR, which comprises the steps of collecting clinical samples from the lung tissue of pigs, extracting DNA from the collected clinical samples, Time multiplex PCR using specific primers that perform gene amplification by performing simultaneous species identification and serotype determination of porcine pleural pneumococcal serotypes 1, 2, 7, and 12 using the DNA template as a template. Speed detection method and apparatus thereof.;