A B C D - PCR Primers and Kits for Colony Multiplex PCR for the Detection of Class A B C and D -lactamase Genes and Methods of Using thereof

摘要

The present invention provides a kit and a primer for a colony multiple PCR capable of detecting Class A, B, C, and D beta-lactamase genes. Rapid observation of the bla gene using the kit and primer according to the present invention enables appropriate antibiotic prescription, which can reduce the patient''s mortality and minimize antibiotic resistance. The present invention exploits (a) early detection of all clinically important bla genes and (b) phenotypic test results using 54 primer pairs designed through a novel and sophisticated optimization process. With perfect specificity and sensitivity in 172 control strains and 403 clinical strains, the present invention provides all bla It provides a rapid clinical application method for identifying genes.