PTO - - Detection of Target Nucleic Acid Sequence by PTO Cleavage and Extension-Dependent Non-Hybridization Assay

摘要

The present invention relates to detection of a target nucleic acid sequence by a PCE-NH2 (PTO cleavage and extension-dependent non-hybridization) assay. The present invention exploits the fact that it inhibits hybridization between CTO and HO due to its formation of a target-dependent extended dimer. Therefore, the present invention can detect the target sequence even when HO is uncut. Regarding PTO, it's 5'-tagging site, CTO and HO sequence It is easy to design, and reaction conditions can also be set easily. Also, between the HO and the CTO it can be carried out in a container that is different from the CTO it extended detection of the hybrid cargo.