FRET Method for detecting nucleic acid based on FRET assay using polymerase lacking exonuclease activity

摘要

The present invention relates to a nucleic acid encoding a target nucleic acid, at least one primer and probe that binds to the target nucleic acid, a deoxynucleotide 5'-triphosphate, and a DNA polymerase that lacks or reduces 5'- 3'exonuclease activity as an enzyme Providing an amplification reaction composition comprising an enzyme and a heat resistant flap endo nuclease; And 5'- 3 'exonuclease activity, which comprises amplifying the target nucleic acid under conditions that allow amplification of the target nucleic acid, using FRET-based nucleic acid detection or A method capable of amplification is disclosed.;