CELL CULTURE METHOD CELL CULTURE DEVICEMETHOD FOR COUNTING SUBJECT MATTERS TO BE COUNTED IN CONTAINER AND DEVICE FOR COUNTING

摘要

By controlling the formation of agglomerates of cells in the culture vessel and controlling the decomposition of agglomerated agglutinates, the cell proliferation efficiency is further improved. It is also possible to measure the number of cells in the culture container without opening the culture system and regardless of the cell density. An external force is applied to the culture container to control the formation of the aggregation mass of the cells in the culture container and the decomposition control of the aggregation mass. The application of the external force to the culture container is carried out by moving the stirring member in the horizontal direction at a predetermined rate by filling the stirring member from the upper surface of the flat culture container with a predetermined amount of indentation so as to form an agglomerated mass of cells in the culture container, /RTI In addition, a method of measuring the number of objects to be counted in the liquid in the sealed container may be a method of adjusting the thickness of at least a part of the container so that at least a part of the adjusted range is used as a measurement object range, Measure the number.