METHOD FOR INTRODUCING PATHOGENIC STREPTOCOCUS GENES INTO CHROMOSOMAL DNA OF PROBIOTIC STRAIN ENTEROCOCCUS FAECIUM L3 FOR EXPRESSION IN PILI

摘要

FIELD: biotechnology.SUBSTANCE: invention relates to a method of producing a live vaccine on the basis of biologically active strain Enterococcus faecium L3 due to the itrogression of the antigen of a clinically relevant pathogenic microorganism into the structure of the strain piles. This method involves producing a fusion gene of entF-bac, consisting of two separate gene fragments of probiotic E.faecium L3 and gene fragment bac and having a nucleotide sequence, shown in fig. 7a, its cloning and detection of bacterial clones expressing the desired protein in piles. The invention also relates to recombinant plasmid DNA pentF-bac. The real DNA pentF-bac is designed to create a live vaccine based on the biologically active strain of E. faecium L3. This pentF-bac DNA is a suicide plasmid pT7ERMB, at BamHI and KpnI sites of which the sequence of entF-bac fusion gene DNA is inserted. The present invention also relates to a vaccine preparation of E.faecium L3 Bac+. This vaccine preparation is prepared by electroporation of E. faecium L3 of plasmid pentF-bac DNA.EFFECT: invention allows for the production of a live vaccine based on the biologically active strain.3 cl, 9 dwg, 1 tbl, 5 ex