PREPARATION, INCREASING PRODUCTION OF NITROGEN OXIDE BY MACROPHAGES IN VITRO, BASED ON HUMIC ACIDS OF PEAT OF TOMSK REGION BOGS AND METHOD FOR OBTAINING THEREOF

摘要

FIELD: medicine.;SUBSTANCE: invention relates to medicine and veterinary. The method for obtaining humic acids, increasing production of nitrogen oxides by macrophages in vitro, from peat of Tomsk region bogs includes crushing initial raw material, treating with exractant with mechanical stirring for 8 hours, sedimentation from solution with inorganic acid, separation of liquid and solid phases and drying the latter, with preliminary drying of peat at room temperature to air-dry state, crushing sieving through sieve with hole diameter 3 mm, with further extraction with sodium pyrophosphate with concentration 2.0-4.0 wt % in mass ratio peat: extractant 1:50-1:100 with constant stirring at temperature 25-27°C; after that extract of humic acids is treated with hydrochloric acid to pH 1-2, sediment of obtained humic acids is separated by centrifugation, after that washed from acidic to pH 7 medium and dried at room temperature. Application of humic acids from peat of Tomsk region bogs to increase nitrogen oxide production by macrophages in vitro.;EFFECT: inventions make it possible to create effective technology of peat processing, which makes it possible to obtain water-soluble humic acids that possess capability to activate secretory properties in macrophages.;2 cl, 3 tbl, 4 ex