REDUCTION OF LEVEL OF LACTATE AND INCREASING OF PRODUCTION OF POLYPEPTIDE BY INHIBITING EXPRESSION OF LACTATE DEHYDROGENASE AND PYRUVATE DEHYDROGENASE KINASE

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology and concerns method of reducing lactate production in mammalian cells, method of silencing or reduction in mammal cell of transcription of lactate dehydrogenase (LDH) and pyruvate dehydrogenase kinase (PDHK), method of producing mammal cells, which exhibits low production of lactate in culture, vector containing first heterologous nucleotide sequence coding small interfering RNA (siRNA), specific for lactate dehydrogenase, and second heterologous nucleotide sequence coding siRNA specific for pyruvate dehydrogenase kinase, each of which is connected with its promoter.;EFFECT: presented inventions allow reducing in cultured cells production of lactate and increasing of production of heterologous polypeptide.;40 cl, 6 dwg