METHOD FOR DETERMINING TOXICITY OF CHEMICALS GENERATING ACTIVE OXYGEN FORMS

摘要

FIELD: chemistry.;SUBSTANCE: method provides adding riboflavin to the final concentration of 1⋅10^-5 mg/ml - 1⋅10^-3 mg/ml into the Escherichia coli K12 MG1655 culture with the pColD-lux plasmid, in which the bioluminescence lux operon of marine photobacteria Photobacterium leiognathi, Vibrio fischeri or Photorabdus luminescens is placed under the control of the pColD promoter. Adding the test substance and determining the luminescence intensity of the resulting suspension and the control are carried out. The damaging effect of the test substance is estimated by the suspension luminescence intensity deviation from the control.;EFFECT: increasing the induction factor.;4 cl, 1 dwg, 2 ex