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METHOD FOR EXPRESS-DIAGNOSTICS OF BLOOD FLOW INFECTION

机译:快速诊断血流感染的方法

摘要

FIELD: medicine.;SUBSTANCE: invention relates to medicine, namely to laboratory methods of research, express-method for diagnostics of infectious disease accompanied by presence of microorganisms in blood. Way of express-method of blood flow infection diagnostics includes identification of microorganisms in blood by method of blood smear microscopy with immersion and magnification ×1000, with smear being prepared from leukocyte layer of venous blood with application of "two glasses" technique. After drying distilled water is poured on smear, covering its entire area, after 3-5 s destructed erythrocytes are washed away and smear is dried. After fixation above burner flame and Gram-staining, microscopy of obtained smear is realised by provision of colourless background of blood smear.;EFFECT: invention ensures increased efficiency of diagnostics of blood flow infection due to possibility of identification of Gram-positive and Gram-negative microorganisms with preservation of simplicity and availability of diagnostics.;1 tbl
机译:药物发明领域本发明涉及医学,即涉及实验室研究方法,用于诊断伴随血液中微生物的传染病的快速方法。血流感染诊断的表​​达方法包括通过浸没和放大倍数×1000的血涂片显微镜法鉴定血液中的微生物,应用“两杯”技术从静脉血的白细胞层制备涂片。干燥后,将蒸馏水倒在涂片上,覆盖整个区域,然后冲洗3-5秒钟破坏的红细胞,然后将涂片干燥。在固定在燃烧器火焰上方并进行革兰氏染色之后,通过提供无色血迹背景来实现获得的涂片的显微镜检查。效果:由于可以鉴定出革兰氏阳性和革兰氏阳性,因此本发明确保了提高的血流感染诊断效率阴性微生物,可简化诊断方法并提供诊断。1tbl

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