METHOD FOR PLAGUE AGENT STRAINS SUBSPECIES DIFFERENTIATION BY PCR WITH REAL TIME HYBRIDIZATION-FLUORESCENT ACCOUNT OF RESULTS

摘要

FIELD: biotechnology.;SUBSTANCE: method for Yersinia pestis plague pathogen strains subspecies differentiation is described. The method involves DNA isolation from the test strain, polymerase chain reaction with real time hybridization-fluorescent account of results with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 primers and SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 probes on 45, 89, Caucasic (-91), His (-205), Alt (-90), Uleg (-88), Tal (-72) DNA target. Yersinia pestis strains subspecies differentiation is performed by the absence of fluorescence signal of the specific subspecies DNA target and its presence on the remaining DNA targets used.;EFFECT: improved method.;1 dwg, 6 ex