METHOD OF CREATION OF KITS OF MICROSPHERES OPTICALLY CODED BY FLUORESCENT NANOCRYSTALS AND CARRYING RECOGNIZING BIOLOGICAL MOLECULES ON THEIR SURFACE

摘要

FIELD: physics.;SUBSTANCE: invention can be used to create sets of microspheres optically coded by depositing on their surface layers of oppositely charged polyelectrolytes and layers of water-soluble fluorescent nanocrystals and bearing on their surface recognizing biological molecules. Optical coding is carried out by applying several layers of fluorescent nanocrystals. A layer of fluorescent nanocrystals of the same colour are spatially separated from the adjacent layer of nanocrystals of a different colour by several layers of polyelectrolytes. To form the outer layer of polyelectrolyte, a polymer is used, which includes the functional groups by which chemical binding reactions are carried out with the functional groups of the recognizing biological molecules. Sets of optically coded microspheres that carry biological molecules of recognizing on their surfaces can be used to simultaneously detect a significant number of disease markers in biological fluids and tissues using flow cytometry techniques.;EFFECT: creation of populations of multilayer microspheres with different spectral optical codes in which each layer of nanocrystals of the same colour is spatially separated by several polyelectrolyte layers from a layer of nanocrystals of a different colour over distances exceeding 10 nm, which avoids effective resonant energy transfer between nanocrystals of different colours.;15 cl, 2 dwg, 3 ex