Romer by weight, the detector and the method for the determination of the radiation exposure by means of chromosome abnormalities

摘要

Method for the detection of radiation exposure of present in a sample chromosomes anomalies, comprising the steps of:(a) determining a position of at least one centromere each chromosome in a cell in an image of a meta phases core by:(i) segmenting an accurately reflect drawn chromosomes means line of each of the chromosomes in the cell,(ii) selecting a longitudinal cross section of a chromosomes image with a minimum width, intensity, or a combination of features, which are derived from width and intensity, wherein in a dicentric chromosome, the location of a first centromere is covered, in order to a following longitudinal cross-section with a second lowest minimum to identify a combined width;(b) determining the number of centromeric in each cell in each chromosome;(c) adding up a calculated frequency dicentric chromosomes in a cell population; andd) determining a radiation dose by comparing the calculated frequency dicentric chromosome to a previously determined dose - action curve from a calibrated ionizing radiation source.