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COMPOSITIONS AND METHODS FOR CONSTRUCTING STRAND SPECIFIC CDNA LIBRARIES
COMPOSITIONS AND METHODS FOR CONSTRUCTING STRAND SPECIFIC CDNA LIBRARIES
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机译:构成特定序列的DNA文库的组合物和方法
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摘要
Provided herein are compositions, kits and methods for the production of strand-specific cDNA libraries. The compositions, kits and methods utilize properties of double stranded polynucleotides, such as RNA-cDNA duplexes to capture and incorporate a novel sequencing adapter. The methods are useful transcriptome profiling by massive parallel sequence, such as full-length RNA sequencing (RNA-Seq) and 3′ tag digital gene expression (DGE).
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