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PURIFICATION OF BACTERIAL CAPSULAR POLYSACCHARIDE BY TWO-PHASE AQUEOUS MICELLAR SYSTEM
PURIFICATION OF BACTERIAL CAPSULAR POLYSACCHARIDE BY TWO-PHASE AQUEOUS MICELLAR SYSTEM
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机译:两相胶束系统纯化细菌性多糖
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摘要
The present invention discloses a method for purification of bacterial capsular polysaccharide at low temperature(20°C) by salt-induced two-phase aqueous micellar system with TritonX-114 and can significantly reduce the endotoxins. Impurity proteins and the endotoxins need to be removed as many as possible in the preparation process of bacterial capsular polysaccharide vaccine to reduce the side reaction of the vaccines. Currently, the phenol extraction method, column chromatography and ethanol precipitation method all have a number of drawbacks. As compared with the phenol, the nonionic detergent has the advantages such as non-corrosiveness and non-carcinogenicity, and does not cause harm to the environment. As compared with the column chromatography, the method of the present invention is large in throughput, time-saving, economical, and easily expanding the scale production, which is a safe, environment-friendly and efficient.
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