首页>
外国专利>
PRIMITIVE GUT ENDODERM CELLS AND METHOD FOR PRODUCING SAME
PRIMITIVE GUT ENDODERM CELLS AND METHOD FOR PRODUCING SAME
展开▼
机译:原始肠内胚层细胞及其生产方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides a technique that serves as a platform for inducing human organ cells at a low cost, stably and in a large quantity. A cell inducible after differentiating pluripotent stem cells and then passaging the resultant cells at least once or more times, which is negative for undifferentiated (pluripotent) cell markers NANOG, OCT4, MYC and LIN28A, negative for endoderm cell markers CXCR4, CER1, HHEX and GATA4, positive for intestinal endoderm cell markers CDX2 and HOXB9, negative for a mesenchymal cell marker brachyury (T), negative for a pancreatic cell marker PDX1, and capable of differentiating into at least a hepatocyte, a pancreatic cell and an intestinal cell. Also provided are methods of preparing and amplifying the above cells; a method of preparing organ cells using the above cells; and a method of constructing a working cell bank for preparing organ cells, comprising cryopreserving the above cells.
展开▼