METHOD FOR THE DETECTION OF DELETIONS, DUPLICATIONS OR INVERSIONS IN THE SAME CRITICAL REGION

摘要

The invention relates to a composition for detecting structural chromosomal aberrations in a sample comprising three probes, wherein a) the first probe comprises a first nucleic acid fragment which is labeled with a first marker, b) the second probe comprises a second nucleic acid fragment which has a second nucleic acid fragment And c) the third probe comprises a third nucleic acid fragment labeled with a third marker, wherein the first, second and third markers are different markers, wherein the first, second and third nucleic acid fragments are each specifically bind a complementary section on a chromosome and the three sections lie on the same chromosome, and wherein the three probes in the specific chromosome bound state distances from each other, by which the three markers can be detected spatially separated from each other and, if and in between the Sections no aberration vorli egt can be detected in the spatial order of first - second - third markers. In addition, the invention also relates to methods for detecting structural chromosome aberrations in a sample, comprising steps of a) contacting a biological sample comprising chromosomes with a composition of the invention under conditions which hybridize the probes to complementary portions allow the chromosomes; and b) proving the spatial order of the three markers.