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A METHOD FOR MEASURING THE PROTEASE ACTIVITY OF FACTOR D OF THE ALTERNATIVE COMPLEMENT PATHWAY
A METHOD FOR MEASURING THE PROTEASE ACTIVITY OF FACTOR D OF THE ALTERNATIVE COMPLEMENT PATHWAY
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机译:一种替代补体途径D因子蛋白酶活性的测定方法
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摘要
A method for measuring the protease activity of Factor D for its natural substrate is provided. Factor D activity may be measured with an immunoassay or a biosensor. The method typically comprises immobilizing a biotinylated C3b on a solid phase coated with a biotin binding protein. Substantially homogeneous components of the alternative complement pathway may be incubated with the immobilized C3b to form a convertase. An immunoassay or a biosensor is generally used to measure the formation of Bb.
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