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MUTANT GENE DETECTION KIT AND MUTANT GENE DETECTION METHOD

机译:突变基因检测试剂盒和突变基因检测方法

摘要

PROBLEM TO BE SOLVED: To provide a kit for detecting mutant genes and a method for detecting mutant genes that can easily and with high sensitivity detect the existence of mutations in a region in which mutations are concentrated.;SOLUTION: The kit for detecting mutant genes is provided with: a probe that, when a plurality of independent regions R1 and R2 that do not overlap in an exon of a target gene are the wild-type base sequence, hybridizes thereto; a label indicating that the probe has hybridized to each of R1 and R2; and a primer pair designed to include all of R1 and R2 in one amplicon obtained by PCR.;SELECTED DRAWING: Figure 1;COPYRIGHT: (C)2018,JPO&INPIT
机译:解决的问题:提供一种用于检测突变基因的试剂盒和一种检测突变基因的方法,该试剂盒和方法可以轻松且高灵敏度地检测突变集中区域中突变的存在。具备:当在靶基因的外显子上不重叠的多个独立区域R1,R2为野生型碱基序列时,与之杂交的探针。指示探针已经与R1和R2中的每一个杂交的标签;引物对设计为在通过PCR获得的一个扩增子中包含所有R1和R2 .;选图:图1;版权:(C)2018,JPO&INPIT

著录项

  • 公开/公告号JP2018113898A

    专利类型

  • 公开/公告日2018-07-26

    原文格式PDF

  • 申请/专利权人 HIROSHIMA UNIV;

    申请/专利号JP20170006625

  • 发明设计人 HIYAMA EIZO;

    申请日2017-01-18

  • 分类号C12N15/09;C12Q1/68;

  • 国家 JP

  • 入库时间 2022-08-21 13:14:03

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