In vivo generation of long double-stranded RNA using VLP

摘要

Generate and isolate virus-like particles (VLPs) containing long sense and antisense RNA molecules, including RNA molecules that are predicted to fold into a double-stranded region longer than the internal diameter of the VLP capsid And methods and compositions for purification are disclosed. A virus-like particle (VLP) containing a capsid encapsulating at least one heterologous cargo molecule and at least one packing sequence, the heterologous cargo molecule containing at least two RNA sequences; Virus-like particles (VLPs), one of which is the reverse complement of the other, each longer than 35 nucleotides. [Selection] Figure 1