Means and methods for minimizing swept volume and dead volume in chromatographic applications

摘要

The present invention relates to a device for preventing spreading of a band and re-mixing of separated fractions comprising a flow selector, for example a chromatography column combined with a rotary valve, and an associated method, wherein said flow The selector is connected to the distal end of said column such that the sum of the post column swept volume and the post column dead volume is less than 10 L. Preferably, the column is directly plugged into the inlet of the rotary valve and the sample is fractionated at the outlet.(FIG.