The present invention provides an easy and simple method for measuring arginase activity with high sensitivity. In the method for measuring arginase activity of the present invention, a sample containing arginase and a solution containing a divalent cation are added to a reaction vessel to activate arginase, and the sample containing activated arginase is added to the reaction vessel. Adding an arginine solution and performing an enzyme substrate reaction; and adding an acidic solution containing a urea detection reagent to a pH of 1.0 or more and 4.0 or less to the sample subjected to the enzyme substrate reaction; And performing the detection reaction at the same time.
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