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Chromatography column packing method, packed chromatography column for use at high pressure, and its use
Chromatography column packing method, packed chromatography column for use at high pressure, and its use
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机译:色谱柱填充方法,用于高压的填充色谱柱及其用途
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摘要
A method of packing a chromatography column, including: dispersing chromatographic media particles in a slurry solution to form a slurry, filling a chromatography column with the chromatographic media particles by introducing the slurry to the column and applying a pressure to pack the chromatographic media particles in the chromatography column. The slurry solution is preferably aqueous based slurry solution. The pressure is preferably applied and held substantially constant for a first period at a first pressure and is applied and held substantially constant for a second period, following the first period, at a second pressure higher than the first pressure.
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