Provided are a compound that generates an SHG signal but has suppressed generation of a TPF signal. Also provided is a cell examination method using the same. A compound for generating a second harmonic of light, the compound being an azobenzene derivative of formula (1), or a salt thereof. (R 1 and R 2 independently represent alkyl groups having 6-12 carbon atoms, and R 3 to R 18 independently represent substituents selected from hydrogen, halogens, alkyl groups, alkoxy groups, aryl groups, amino groups, hydroxyl groups, nitro groups, and cyano groups, R 5 and R 6 , R 9 and R 10 , R 13 and R 14 , and R 17 and R 18 may combine together and form a ring structure having 5-7 carbon atoms. X represents -N + R 19 R 20 R 21 , a sulfonyl group, a carboxyl group, or an -OR group. Here, R 19 , R 20 , and R 21 independently represent straight-chain or branched alkyl groups having 1-5 carbon atoms, and -OR represents a monovalent polyalkylene oxide group terminated by an alkoxy group. a is 0 or 1, b is 0 or 1, and n is an integer of 1-10.)
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机译:提供了一种产生SHG信号但抑制了TPF信号产生的化合物。还提供了使用该细胞检查方法的细胞检查方法。用于产生二次谐波的化合物或其盐是式(1)的偶氮苯衍生物。 (R 1和R 2独立地表示具有6-12个碳原子的烷基,并且R 3至R 18独立地表示选自氢,卤素,烷基,烷氧基,芳基,氨基,羟基,硝基的取代基,氰基,R 5和R 6,R 9和R 10,R 13和R 14以及R 17和R 18可以结合在一起形成具有5-7个碳原子的环结构,X表示-N + R 19 R 20 R 21,磺酰基,羧基或-OR基,其中,R 19,R 20和R 21独立地表示碳原子数为1〜5的直链或支链烷基,-OR表示被烷氧基封端的一价聚环氧烷基。a为0或1,b为0或1,n为1-10的整数。)
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