Antibody-binding phosphor-integrated nanoparticles, method for producing antibody-binding phosphor-integrated nanoparticles, and immunostaining kit

摘要

[Problem] The present invention has an object to provide antibody-binding phosphor-integrated nanoparticles, a method for producing antibody-binding phosphor-integrated nanoparticles, and an immunostaining reagent kit capable of suppressing a decrease in staining efficiency of immunostaining due to molecular aggregation. To do. [Solution] An SH group generated by reducing an antibody and phosphor-integrated nanoparticles by reducing a disulfide bond (-SS-) of the antibody; and a binding group on the surface of the phosphor-integrated nanoparticles; The antibody-bound phosphor-aggregated nanoparticles bound by the reaction of (2), wherein streptavidin in which disulfide bonds are reduced to SH groups using 2-iminothiolane is within the unit area of the surface of the phosphor-aggregated nanoparticles. An antibody-binding phosphor, wherein when the number that can be bound to a predetermined number of binding groups is expressed as nmol, the number of the antibodies bound to the predetermined number of binding groups is 2 nmol or more. Integrated nanoparticles.