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METHOD CAPABLE OF BEING USED FOR PROTEIN RENATURATION INDEPENDENTLY OR BEING USED AS PRECEDING OPERATIONS OF PROTEIN RENATURATION
METHOD CAPABLE OF BEING USED FOR PROTEIN RENATURATION INDEPENDENTLY OR BEING USED AS PRECEDING OPERATIONS OF PROTEIN RENATURATION
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机译:可以独立用于蛋白质重新命名的方法,或者可以用作蛋白质重新命名的先决条件的方法
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摘要
Provided is a method of treating a protein to be renatured, including: (1) mixing the protein to be renatured with a denaturing solution containing a denaturing reagent; (2) incubating a resulting mixture at such a low temperature that the denaturing agent is gradually precipitated from the denaturing solution, resulting in a decreasing concentration gradient of the denaturing agent and an increasing concentration of a renatured protein or its precursor in the denaturing solution with a decreasing volume; and (3) obtaining at least one of the renatured protein and its precursor.
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