Novel Genetic Code and Translation System for Producing Polypeptide, and Method for Producing Polypeptide

摘要

The present invention provides a translation system for expressing a polypeptide having a predetermined amino acid sequence according to a code different from the universal genetic code, comprising a template nucleic acid having a sequence modified from a nucleic acid sequence encoding the polypeptide by interchanging codons encoding at least 2 amino acids selected from serine, leucine, and alanine; and at least either (i) or (ii):(i) an aminoacyl-tRNA having an anticodon to a codon after interchange and to which an amino acid encoded by a codon before the interchange is bound;(ii) a tRNA having an anticodon to a codon after interchange and recognized by an aminoacyl-tRNA synthetase corresponding to an amino acid encoded by a codon before the interchange, or a nucleic acid encoding the tRNA, the amino acids, and the aminoacyl-tRNA synthetase or a nucleic acid encoding the aminoacyl-tRNA synthetase.