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THERMAL CYCLING METHODS AND APPARATUSES FOR CARRYING OUT EFFICIENT POLYMERASE CHAIN REACTION (PCR) PROCESSES TO AMPLIFY DEOXYRIBONUCLEIC ACID (DNA)
THERMAL CYCLING METHODS AND APPARATUSES FOR CARRYING OUT EFFICIENT POLYMERASE CHAIN REACTION (PCR) PROCESSES TO AMPLIFY DEOXYRIBONUCLEIC ACID (DNA)
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机译:进行高效聚合酶链反应(PCR)扩增脱氧核糖核酸(DNA)的热循环方法和装置
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摘要
A thermal cycling method and associated device is described. The method is for carrying out a polymerase chain reaction (PCR) process to amplify deoxyribonucleic acid (DNA), and the method includes: pre-heating a series of blocks to respective temperatures that correspond to different respective heating stages in a PCR process, in which each block of the series of blocks defines a respective heat transfer surface, in which the series of blocks define a sequence of positions along a path, with each position defined by a respective heat transfer surface of a respective block; and moving a PCR reaction vessel, which contains deoxyribonucleic acid (DNA) and PCR reagents, along the path into and out of each respective position in the sequence of positions according to a schedule, in which, at each respective position the PCR reaction vessel is in thermal contact with the respective heat transfer surface to equilibrate a temperature of the PCR reaction vessel to a target temperature that corresponds to a respective heating stage in the PCR process.
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